The exigo H400 system is an automated hematology analyzer for in vitro diagnostic use under laboratory conditions (Fig. 1). The analyzer is intended for determination of hemoglobin (HGB) concentration, for counting of red blood cells (RBC) and platelets (PLT), as well as for counting and differentiation of white blood cells (WBC) into four subpopulations, namely lymphocytes (LYM), monocytes (MONO), neutrophils (NEU), and eosinophils (EOS). The measurement principles of the exigo H400 are based on impedance for cell counts and spectrophotometry for HGB.
This study validates the performance of exigo H400 against a reference system for dog, cat and horse, using normal and abnormal fresh whole blood samples collected from patients for routine analysis.
Comparison of test and reference systems
The performance of the exigo H400 4-part hematology analyzer (test system) was compared to that of the Sysmex™ XT-2000iV 5-part hematology analyzer (reference system). Exigo H400 operates with impedance technology whereas the reference analyzer uses fluorescence flow cytometry. The results from this study demonstrate that the performance of exigo H400 hematology analyzer is in good agreement with that of the reference analyzer and the manual reference method, indicating the suitability of exigo H400 for use in routine hematology analysis at veterinary clinics.