The reagent composition is of utmost importance for reliable results. The Diluent provides an isotonic environment for the RBCs and PLTs, while the Lyse reagent is capable of lysing the RBCs to release HGB and shrink the WBCs to allow differentiation of these cells into their subgroups.
For Swelab Alfa Plus analyzer only two reagents—Diluent and Lyse—is required and for Swelab Lumi three reagents are required – Diluent, Lyse 1, and Lyse 2. This is greatly facilitates handling and logistics and helps reduce running costs. Simply scan the RFID card on the reagent container and the analyzer stores key information such as lot number, open and expiry dates, and remaining volume.
To avoid reagent shortage and to ensure an exact sample dilution each time, Boule adds a small extra volume to each reagent container. To prevent air from entering the system, the small volume that is left in the container when all cycles are consumed should not be used.